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wheat germ agglutinin wga  (Vector Laboratories)


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    Structured Review

    Vector Laboratories wheat germ agglutinin wga
    Wheat Germ Agglutinin Wga, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 267 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wheat+germ+agglutinin+wga/Rhodamine+labeled+Wheat+Germ+Agglutinin+(WGA)/pm42037405-280-9-17
    Average 96 stars, based on 267 article reviews
    wheat germ agglutinin wga - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Control:

    Article Title: Concanavalin A targets phylogenetically conserved N-linked glycans on coronavirus spike proteins for broad-spectrum antiviral activity.
    Article Snippet: Concanavalin A used in this study was commercially purchased from Sigma-Aldrich (C0412-5MG) and was dissolved in PBS. .. Control lectins such as peanut agglutinin (PNA) (B-1075-5) and wheat germ agglutinin (WGA) (RL-1022-5) were purchased from Vector Laboratory. .. Recombinant glycosidase Endo H (P0702L) and PNGase F (P0704L) were purchased from New England Biolabs and were used according to the manufacturer’s instructions prior to pulldown assays.

    Whole Genome Amplification:

    Article Title: Concanavalin A targets phylogenetically conserved N-linked glycans on coronavirus spike proteins for broad-spectrum antiviral activity.
    Article Snippet: Concanavalin A used in this study was commercially purchased from Sigma-Aldrich (C0412-5MG) and was dissolved in PBS. .. Control lectins such as peanut agglutinin (PNA) (B-1075-5) and wheat germ agglutinin (WGA) (RL-1022-5) were purchased from Vector Laboratory. .. Recombinant glycosidase Endo H (P0702L) and PNGase F (P0704L) were purchased from New England Biolabs and were used according to the manufacturer’s instructions prior to pulldown assays.

    Article Title: Targeting Siglec–sialic acid checkpoint to reverse immune dysfunction mediated by plasmacytoid dendritic cells in myeloma
    Article Snippet: .. Biotinylated Concanavalin A (Con A), Galanthus nivalis agglutinin (GNA), wheat germ agglutinin (WGA), Maackia amurensis lectin (MAL-II) and Sambucus nigra lectin (SNA) were from Vector Laboratories. .. Streptavidin-conjugated antibodies were from BioLegend.

    Article Title: Glucosamine induces apoptosis of cholangiocarcinoma cells by suppressing high-mannose type N -glycosylation and EGFR/STAT3 signaling
    Article Snippet: .. The membranes were blocked for nonspecific binding with 5% bovine serum albumin (w/v) in TBST for 1 h at room temperature, and then incubated with 0.25 μg/mL biotinylated lectins as follows; concanavalin A (Con A) (Vector Laboratories, Newark, NJ), wheat germ agglutinin (WGA) (Vector Laboratories), and Phaseolus Vulgaris erythroagglutinin (PHA-E) (Vector Laboratories), for 1 h at room temperature. .. The membranes were washed with TBST for 10 min, three times, and incubated with streptavidin-horseradish peroxidase conjugate (Invitrogen, Frederick, MD) for 1 h at room temperature.

    Article Title: Regulatory roles of three Zn(II)2Cys6 cluster-coding genes in insect pathogenicity and environmental fitness of Beauveria bassiana
    Article Snippet: Numerous transcription factors (TFs) with Zn(II)2Cys6 binuclear cluster DNA-binding (C6 or GAL4) domain are generally underexplored in filamentous fungi.. Here, 202 C6 TFs with GAL4 domain are verified in Beauveria bassiana and sorted into three subclasses with distinctive domain architectures.. Three representative C6 TFs (138, 359, and 1117 aa) with an N-terminal GAL4 domain alone were proven to be regulators of insect pathogenicity, named Rip1–3.

    Article Title: MCD enzyme deficiency drives early mortality and multiorgan metabolic disruption in Dahl salt-sensitive rats
    Article Snippet: .. Heart sections were stained with wheat germ agglutinin (WGA) (Cat# RL-1022; Vector Laboratories, Burlingame, CA) to delineate cell membranes and counterstained with Hoechst (Cat# R37165 , Thermo Fisher Scientific) to visualize nuclei. ..

    Article Title: (Pro)renin receptor (PRR) exacerbates diabetic cardiomyopathy by suppressing LRRK2-Mediated mitophagy and promoting senescence.
    Article Snippet: Background: Diabetic cardiomyopathy (DCM) is a major complication of diabetes mellitus, leading to significant mortality.. The (Pro)renin Receptor (PRR) is implicated in cardiovascular pathology, but its specific role in regulating mitochondrial quality control and cellular senescence in the context of DCM remains poorly understood.. This study aimed to elucidate the mechanism by which PRR contributes to myocardial injury in DCM.

    Article Title: Single-cell mapping of the glycocalyx in the adult human and mouse ocular surface
    Article Snippet: Lectins were conjugated to DNA oligonucleotides, each carrying a unique barcode sequence for precise identification and a capture sequence compatible with the 10x Genomics Next GEM kit. .. Unconjugated lectins—including AAL, Maclura pomifera agglutinin (MPA), Maackia amurensis agglutinin I (MAA I), Concanavalin A (ConA), Datura stramonium agglutinin (DSA), wheat germ agglutinin (WGA), peanut agglutinin (PNA), and Erythrina cristagalli agglutinin (ECA)—as well as biotinylated Sambucus nigra agglutinin I (SNA I), were obtained from Vector Laboratories (Newark, CA, USA). .. DNA oligonucleotides for lectin conjugation were synthesized by Integrated DNA Technologies (Coralville, IA, USA).

    other:

    Article Title: Single-cell mapping of the glycocalyx in the adult human and mouse ocular surface
    Article Snippet: DNA oligonucleotides for lectin conjugation were 21 synthesized by Integrated DNA Technologies (Coralville, IA, USA).

    Binding Assay:

    Article Title: Glucosamine induces apoptosis of cholangiocarcinoma cells by suppressing high-mannose type N -glycosylation and EGFR/STAT3 signaling
    Article Snippet: .. The membranes were blocked for nonspecific binding with 5% bovine serum albumin (w/v) in TBST for 1 h at room temperature, and then incubated with 0.25 μg/mL biotinylated lectins as follows; concanavalin A (Con A) (Vector Laboratories, Newark, NJ), wheat germ agglutinin (WGA) (Vector Laboratories), and Phaseolus Vulgaris erythroagglutinin (PHA-E) (Vector Laboratories), for 1 h at room temperature. .. The membranes were washed with TBST for 10 min, three times, and incubated with streptavidin-horseradish peroxidase conjugate (Invitrogen, Frederick, MD) for 1 h at room temperature.

    Incubation:

    Article Title: Glucosamine induces apoptosis of cholangiocarcinoma cells by suppressing high-mannose type N -glycosylation and EGFR/STAT3 signaling
    Article Snippet: .. The membranes were blocked for nonspecific binding with 5% bovine serum albumin (w/v) in TBST for 1 h at room temperature, and then incubated with 0.25 μg/mL biotinylated lectins as follows; concanavalin A (Con A) (Vector Laboratories, Newark, NJ), wheat germ agglutinin (WGA) (Vector Laboratories), and Phaseolus Vulgaris erythroagglutinin (PHA-E) (Vector Laboratories), for 1 h at room temperature. .. The membranes were washed with TBST for 10 min, three times, and incubated with streptavidin-horseradish peroxidase conjugate (Invitrogen, Frederick, MD) for 1 h at room temperature.

    Labeling:

    Article Title: Regulatory roles of three Zn(II)2Cys6 cluster-coding genes in insect pathogenicity and environmental fitness of Beauveria bassiana
    Article Snippet: Numerous transcription factors (TFs) with Zn(II)2Cys6 binuclear cluster DNA-binding (C6 or GAL4) domain are generally underexplored in filamentous fungi.. Here, 202 C6 TFs with GAL4 domain are verified in Beauveria bassiana and sorted into three subclasses with distinctive domain architectures.. Three representative C6 TFs (138, 359, and 1117 aa) with an N-terminal GAL4 domain alone were proven to be regulators of insect pathogenicity, named Rip1–3.

    Fluorescence:

    Article Title: Regulatory roles of three Zn(II)2Cys6 cluster-coding genes in insect pathogenicity and environmental fitness of Beauveria bassiana
    Article Snippet: Numerous transcription factors (TFs) with Zn(II)2Cys6 binuclear cluster DNA-binding (C6 or GAL4) domain are generally underexplored in filamentous fungi.. Here, 202 C6 TFs with GAL4 domain are verified in Beauveria bassiana and sorted into three subclasses with distinctive domain architectures.. Three representative C6 TFs (138, 359, and 1117 aa) with an N-terminal GAL4 domain alone were proven to be regulators of insect pathogenicity, named Rip1–3.

    Flow Cytometry:

    Article Title: Regulatory roles of three Zn(II)2Cys6 cluster-coding genes in insect pathogenicity and environmental fitness of Beauveria bassiana
    Article Snippet: Numerous transcription factors (TFs) with Zn(II)2Cys6 binuclear cluster DNA-binding (C6 or GAL4) domain are generally underexplored in filamentous fungi.. Here, 202 C6 TFs with GAL4 domain are verified in Beauveria bassiana and sorted into three subclasses with distinctive domain architectures.. Three representative C6 TFs (138, 359, and 1117 aa) with an N-terminal GAL4 domain alone were proven to be regulators of insect pathogenicity, named Rip1–3.

    Staining:

    Article Title: MCD enzyme deficiency drives early mortality and multiorgan metabolic disruption in Dahl salt-sensitive rats
    Article Snippet: .. Heart sections were stained with wheat germ agglutinin (WGA) (Cat# RL-1022; Vector Laboratories, Burlingame, CA) to delineate cell membranes and counterstained with Hoechst (Cat# R37165 , Thermo Fisher Scientific) to visualize nuclei. ..



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